
Thermo Fisher Scientific KRT19 Monoclonal Antibody (OTI5D1), TrueMAB
KRT19 단백질을 인식하는 Mouse Monoclonal 항체로, Western blot, IHC, ICC 등 다양한 응용에 사용 가능. Human 및 Dog 시료에 반응하며, Lyophilized 형태로 제공. 고순도의 Affinity chromatography 정제 방식 적용. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5D1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 240–390 of human CK19 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Cytokeratin 19 (CK19) is part of the intermediate filament protein family, exhibiting biochemical diversity across human epithelial tissues with at least 20 distinct polypeptides.
Cytokeratins range in molecular weight from 40–68 kDa and pI 4.9–7.8. Their expression patterns are characteristic of epithelial type and differentiation stage, aiding in tumor classification and diagnosis.
CK19 is not expressed in hepatocytes, making it useful for identifying liver metastases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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