Thermo Fisher Scientific VEGF-165 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF 165. Anti-Human VEGF 165-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human VEGF 165 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human VEGF 165 (500-P10) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human VEGF 165.

Western Blot:
To detect hVEGF by Western blot, this antibody can be used at 0.1–0.2 µg/mL.
With compatible secondary reagents, detection limit for Recombinant hVEGF is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P10BT-1MG supplied as 2 × 500 µg.

Target Information

The vascular endothelial growth factor (VEGF) family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF.
VEGF and its receptor system are key regulators of angiogenesis signaling.
Most tumors require angiogenesis, and VEGF is the most potent angiogenic cytokine associated with this process.
Five isoforms of VEGF are generated by alternative splicing from a single gene and bind to tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), expressed mainly on endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.