Thermo Fisher Scientific VEGF-165 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF-165. Anti-Human VEGF-165-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human VEGF-165 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human VEGF-165 (500-P10) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human VEGF-165.

Western Blot:
To detect hVEGF by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant hVEGF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package:
500-P10BT-1MG is provided as 2 × 500 µg.

Target Information

The vascular endothelial growth factor (VEGF) family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system are key regulators of angiogenesis signaling. Most tumors require angiogenesis, and VEGF is the most potent angiogenic cytokine involved in this process.
Five isoforms of VEGF are generated by alternative splicing from a single VEGF gene. These isoforms bind to two tyrosine-kinase receptors, flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), expressed primarily on endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.