Thermo Fisher Scientific V5 Tag Recombinant Mouse Monoclonal Antibody (SV5-P-K)
V5 태그 단백질 검출용 마우스 단클론 항체로 다양한 어플리케이션(WB, IHC, ICC, ELISA 등)에 사용 가능. HEK293 세포에서 발현된 재조합 항체이며 Protein A로 정제됨. 고순도 액상 형태로 제공되며 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1,000 ng/mL |
| Immunoprecipitation (IP) | Assay-dependent |
| Radioimmune Assays (RIA) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SV5-P-K |
| Immunogen | Pk (V5) Epitope Tag (GKPIPNPLLGLDST) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074236 |
Product Specific Information
Specificity: Recognizes a 14 amino acid sequence (GKPIPNPLLGLDST) derived from the P and V proteins of the paramyxovirus simian virus 5.
Target Information
Epitope tags provide a method to localize gene products in various cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein-specific antibodies are not available.
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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