Thermo Fisher Scientific EGFR Chimeric Recombinant Rabbit Monoclonal Antibody (528)
EGFR 단백질의 세포외 도메인을 인식하는 Thermo Fisher의 재조합 토끼 단일클론 항체입니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 분석에 사용 가능하며, HEK293 세포에서 발현된 비결합형 항체입니다. 단기 4°C, 장기 -20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
| Immunoprecipitation (IP) | Assay-dependent |
| Neutralization (Neu) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 528 |
| Immunogen | Partially purified EGFR from A431 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074231 |
Product Specific Information
Specificity: Recognizes the extracellular domain of EGFR.
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression of EGFR is associated with numerous cancers and serves as a target for biological and small molecule therapeutics. The EGFR gene is located on chromosome 7 in humans and belongs to the HER/ERbB family, which includes ERbB2, ERbB3, and ERbB4. EGFR is a transmembrane receptor; ligand binding (e.g., EGF, TGF-alpha) induces dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain. Overexpression has been observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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