
Thermo Fisher Scientific KRT8 Monoclonal Antibody (OTI1B12), TrueMAB
KRT8 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC/IF에 적합합니다. 인간, 개, 랫드에 반응하며, 동결건조 형태로 제공됩니다. 세포 구조 연구 및 단백질 발현 분석에 활용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2500–1:5000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:150 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B12 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 91–381 of human CK8 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Cytokeratin 8 (CK8) is a member of the type II keratin family located on the long arm of chromosome 12. Type I and type II keratins form heteropolymers that create intermediate-sized filaments in epithelial cells.
CK8 typically pairs with keratin 18 to form filaments in simple epithelial tissues, contributing to cellular structural integrity, signal transduction, and differentiation.
Mutations in this gene are associated with cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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