Thermo Fisher Scientific Actin Recombinant Rabbit Monoclonal Antibody (JF47-01)
Recombinant rabbit monoclonal antibody targeting Actin (JF47-01). Validated for WB, IHC, ICC/IF. High specificity and lot-to-lot consistency. Suitable for human, mouse, rat, and zebrafish samples. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF47-01 |
| Immunogen | Synthetic peptide within Human Actin (aa 303–349) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809807 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Actin is a ubiquitous protein forming filaments that constitute major portions of the cytoskeleton. It interacts with myosin to assist in muscle contraction, cell motility, and cytokinesis.
In vertebrates, actin isoforms are categorized into three groups:
- Alpha actins: Found in muscle tissues; key in the contractile apparatus
- Beta and gamma actins: Present in most cell types; involved in cytoskeleton structure and internal motility
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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