
Thermo Fisher Scientific Galectin 3 Recombinant Rabbit Monoclonal Antibody (JF39-10)
Galectin 3 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 높은 특이성과 재현성을 제공하며, Protein A로 정제된 액상 형태. 인체 및 랫트 시료에 반응하며 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF39-10 |
| Immunogen | Synthetic peptide within Human Galectin 3 aa 1–49 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809804 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Galectin 3 is a galactose-specific lectin that binds IgE. It may mediate endothelial cell migration through interaction with alpha-3, beta-1 integrin and CSPG4. Together with DMBT1, it is required for terminal differentiation of columnar epithelial cells during early embryogenesis.
In the nucleus, Galectin 3 acts as a pre-mRNA splicing factor and participates in acute inflammatory responses, including neutrophil activation and adhesion, monocyte/macrophage chemoattraction, opsonization of apoptotic neutrophils, and mast cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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