Thermo Fisher Scientific Histone H2A Polyclonal Antibody
Rabbit polyclonal antibody recognizing total Histone H2A, H2A.X, and H2A.Z. Validated for WB, IHC(P), and IP. Suitable for human, mouse, rat, and primate samples. Supplied as liquid, Protein A purified, in HEPES buffer with 50% glycerol. For research u...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunoprecipitation (IP) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide based on the region around Threonine 77 of human Histone H2A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 10 µg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10978130 |
Product Specific Information
This antibody detects endogenous levels of total histone H2A protein. It also cross-reacts with histone H2A.X and histone H2A.Z.
It is not recommended to aliquot this antibody.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes.
Two molecules each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and functions in chromatin compaction into higher-order structures.
This gene is intronless and encodes a member of the histone H4 family. Transcripts lack polyA tails but contain a palindromic termination element.
This gene is located in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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