Thermo Fisher Scientific LC3B Polyclonal Antibody, HRP
LC3B 단백질을 인식하는 HRP 결합 폴리클로날 항체로, Western blot, IHC, ELISA 등 다양한 응용에 적합합니다. 인간 LC3B에 특이적이며, 자가포식 연구에 활용됩니다. 항원 친화 크로마토그래피로 정제된 고순도 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| ELISA | Assay-Dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Avian, Bacteria, Bovine, Dog, Chicken, Guinea pig, Hamster, Human, Invertebrate, Mouse, Non-human primate, Pig, Rabbit, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1–100). |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 0.92 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_11156186 |
Product Specific Information
This antibody has 92% homology with mouse, 91% with bovine, and 84% with xenopus.
Reacts weaker with mouse than with human samples.
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B). LC3B is associated with microtubule assembly and plays an important role in neurogenesis.
LC3B is a key protein in the autophagy process, which involves the degradation of cytoplasmic components. Three human LC3 isoforms undergo post-translational modifications during autophagy. Macroautophagy is the major inducible pathway for turnover of cytoplasmic constituents and degradation of enzymes and organelles during nutrient starvation.
LC3B mediates physical interactions between microtubules and cytoskeletal components. It may play roles in cancer, aging, metabolic and neurodegenerative disorders, as well as cardiovascular and pulmonary diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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