Thermo Fisher Scientific GFP Polyclonal Antibody, Alexa Fluor 594

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

Assay-dependent

View 15 publications 15 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1-10 µg/mL

View 11 publications 11 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The GFP was isolated directly from the jellyfish Aequorea victoria.

Conjugate

Alexa Fluor™ 594 Alexa Fluor™ 594 Alexa Fluor™ 594

Excitation/Emission Max

590/618 nm View spectra

Form

Liquid

Concentration

2 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_221478

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.