Thermo Fisher Scientific Phosphatidylinositol 3,4,5-trisphosphate Monoclonal Antibody (RC6F8)

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 6 publications 6 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

RC6F8

Immunogen

phosphatidylinositol 3,4,5-diphosphate

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2, with 0.1% BSA

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2535837

Target Information

Phosphatidylinositol (3, 4, 5)-trisphosphate (PtdIns(3, 4, 5)P3) commonly abbreviated to PIP3 is the product of the class I phosphoinositide 3-kinases (PI 3-kinases) activity on phosphatidylinositol (4, 5)-bisphosphate. PtdIns (3, 4, 5) P3 is dephophosphorylated by the phosphatase, PTEN on the 3 position and by SHIPs (SH2-containing inositol phosphatase) on the 5` position of the inositol ring. The PH domain in a number of proteins binds to PtdIns (3, 4, 5) P3. Such proteins include Akt/PKB, PDK1, Btk1 and ARNO. The generation of PtdIns (3, 4, 5) P3 at the plasma membrane upon the activation of class I PI 3-kinases causes these proteins to translocate to the plasma membrane and accordingly affects their activity. The PH domain allows binding between PtdIns (3, 4, 5) P3 and G-protein coupled receptor kinases (GRKs). This enhances the binding of the GRK to the plasma membrane. [National Center for Biotechnology Information. PubChem Compound Database; CID=53477782].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.