Thermo Fisher Scientific GFP Polyclonal Antibody, Alexa Fluor 488

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

1:200-1:1,000

View 202 publications 202 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 16 publications 16 publications

Immunohistochemistry - Free Floating (IHC (Free))

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1-10 µg/mL

View 58 publications 58 publications

Flow Cytometry (Flow)

-

View 16 publications 16 publications

ChIP assay (ChIP)

-

View 1 publication 1 publication

Inhibition Assays (IA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 11 publications 11 publications

Product Specifications

Species Reactivity

Tag

Published species

Mouse, Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The GFP was isolated directly from the jellyfish Aequorea victoria.

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

2 mg/mL

Purification

IgG fraction

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_221477

Product Specific Information

To achieve appropriate staining results on formalin-fixed, paraffin embedded tissue sections, protease Type XIV (Actinase E) pretreatment is required. The recommended dilution and incubation for protease is 1-2 mg/mL at 37°C for 10 minutes. Other enzyme and Heat Induced Epitope Retrieval pre-treatment methods may not be suitable for this particular antibody. Frozen sections have been successfully stained after fixation with acetone at 4°C.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.