Thermo Fisher Scientific PKM1/PKM2 Monoclonal Antibody (F.313.7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Immunoprecipitation (IP)

2-5 µL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

F.313.7

Immunogen

Synthetic peptide corresponding to the sequence of human PKM1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

43 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_11005173

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.