Thermo Fisher Scientific TSC1 Monoclonal Antibody (OTI3A2), TrueMAB

Thermo Fisher Scientific TSC1 Monoclonal Antibody (OTI3A2), TrueMAB

Applications and Tested Dilution

• Western Blot (WB): 1:2,000
• Immunohistochemistry (Paraffin) (IHC (P)): 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either the TSC1 or TSC2 tumor suppressor genes. These gene products form a protein complex that negatively regulates the mammalian Target of Rapamycin (TOR) pathway, which controls cell growth and cycle progression.
The TSC1/TSC2 complex stimulates Rheb GTPase activity, thereby reducing TOR activation. Overexpression of TSC1/TSC2 decreases the GTP/GDP ratio bound to Rheb, inhibiting cell proliferation. A shorter 40 kDa isoform of TSC1 has been identified, though its function remains unknown.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.