Thermo Fisher Scientific Phosphoserine/threonine Polyclonal Antibody
Western blot과 ELISA에 적합한 rabbit polyclonal antibody로, phosphoserine 및 phosphothreonine 잔기를 인식합니다. 합성 인산화 펩타이드를 면역원으로 사용하였으며, 세포 신호전달 경로 연구에 유용합니다. 액상 형태로 제공되며 -20°C에서 보관합니다.
- 판매단위
- pk
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
ELISA
- Tested Dilution: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence containing phosphorylated S & T |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.49 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914886 |
Product Specific Information
Positive test controls include: C6.
Target Information
Signal transduction pathways often involve reversible phosphorylation of serine and threonine residues within specific motifs of target proteins. These motifs are recognized by protein kinases and phosphorylation-dependent binding proteins such as 14-3-3. Such domains are crucial for modulating and amplifying intracellular communication.
Key kinases like Akt, RSK1, p70 S6K, and PKC isoforms are regulated by phosphorylation at serine or threonine sites surrounded by phenylalanine or tyrosine residues, which are essential for kinase activity.
The (Ser/Thr) Phe Antibody serves as a powerful tool for identifying proteins containing these regulatory motifs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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