Thermo Fisher Scientific CCND1 Monoclonal Antibody (OTI8B2), TrueMAB

Applications

• Western Blot (WB): 1:2,000 dilution tested

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Cyclin D1 (PRAD-1, bcl-1) is a key regulator of the cell cycle, functioning with CDK4 and/or CDK6 to phosphorylate the Rb protein. It is a proto-oncogene overexpressed in various human cancers, including mantle cell lymphoma. Cyclin D1 regulates protein phosphorylation, epithelial cell proliferation, and adipocyte differentiation.

The CCND1 gene is located on chromosome 11 and encodes a cytoplasmic and nuclear protein synthesized during the G1 phase. Cyclin D1-CDK complexes inactivate Rb through phosphorylation and modulate CDK inhibitors such as p21^Cip1^ and p27^Kip1^. Without growth factor stimulation, Cyclin D1 undergoes ubiquitin-mediated degradation, leading to G1/S arrest.

Cyclin D1 belongs to the conserved cyclin family, characterized by periodic changes in protein levels during the cell cycle. It forms complexes with CDK4 or CDK6, essential for G1/S transition, and interacts with tumor suppressor Rb. Overexpression or mutation of this gene is associated with tumorigenesis, making it a valuable target for cell cycle and cancer research.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.