Thermo Fisher Scientific Phospho-RNA pol II CTD (Ser2) Recombinant Rabbit Monoclonal Antibody (7H23L1)
Phospho-RNA pol II CTD (Ser2)를 인식하는 재조합 토끼 단클론 항체로, Western blot, ICC/IF, ChIP, CUT&RUN에 적합합니다. 높은 특이성과 재현성을 제공하며, 사람 및 생쥐 시료에 반응합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | 2 µg/million cells |
| CUT&RUN (C&R) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7H23L1 |
| Immunogen | Peptide corresponding to human Phospho - POLR2A (Ser2) [1-8] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2784561 |
Product Specific Information
This antibody is predicted to react with Monkey and Rat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed, and individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
RNA polymerase II is the enzyme responsible for synthesizing mRNA and many non-coding RNAs. The POLR2A gene encodes the RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II.
The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats (YSPTSPS). Phosphorylation and other modifications within this sequence regulate the recruitment of factors involved in the transcription cycle.
Phosphorylation of Serine-2 and Serine-5 residues within the CTD coordinates the recruitment of RNA processing factors to the elongation complex.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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