Thermo Fisher Scientific TSC1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody recognizes both isoforms of TSC1.
A suggested positive control is EL4 cell lysate.
PA5-20131 can be used with blocking peptide PEP-0249.

Target Information

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes.
The products of these genes form a protein complex that indirectly decreases signaling of the mammalian Target of Rapamycin (TOR), a serine/threonine kinase regulating cell growth and cycle through nutrient and growth factor signals.
TOR activity is stimulated by Rheb, a Ras superfamily G-protein, when the GTP/GDP ratio bound to Rheb is high.
Immunoprecipitated TSC1/TSC2 stimulates Rheb GTPase activity in vitro, decreasing Rheb-mediated TOR activation.
Overexpression of TSC1 and TSC2 leads to a reduced GTP/GDP ratio bound to Rheb and inhibition of cell growth.
A shorter 40 kDa isoform of TSC1 also exists, though its function remains unknown.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.