Thermo Fisher Scientific Aequorea victoria GFP His-tag Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• Immunohistochemistry (IHC): Assay-dependent
• ELISA: Assay-dependent
• Functional Assay: Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Product Specific Information

Reconstitution:
Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP is widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.

As a fusion tag, GFP can be used to localize proteins, study their movement, or research the dynamics of subcellular compartments. GFP technology has provided significant insights into the physiological activities of living cells.

GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (max. 395 nm) and emits green light (peak 508 nm) without additional proteins or cofactors. GFP fluorescence is stable, species-independent, and suitable for various applications such as monitoring gene expression, protein localization, and protein–protein interactions (e.g., yeast two-hybrid system, FRET experiments).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.