Thermo Fisher Scientific Aequorea victoria GFP His-tag Recombinant Protein
Aequorea victoria 유래 GFP His-tag 재조합 단백질로, 형광 표지 및 단백질 위치 추적 연구에 적합. E. coli 발현 시스템에서 정제된 고순도 단백질. 다양한 어세이(WB, IHC, ELISA 등)에 활용 가능. 연구용으로만 사용.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Cnidarian |
| Expression System | E. coli |
| Amino Acid Sequence | Aequorea victoria GFP, amino acids Ser2–Lys238 (Accession # AAB65663) with an N-terminal His-tag |
| Tag | His-tag |
| Molecular Weight | 28.7 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥90% (SDS-PAGE), ≥85% (SEC-HPLC) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient |
Product Specific Information
Reconstitution:
Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP is widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP can be used to localize proteins, study their movement, or research the dynamics of subcellular compartments. GFP technology has provided significant insights into the physiological activities of living cells.
GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (max. 395 nm) and emits green light (peak 508 nm) without additional proteins or cofactors. GFP fluorescence is stable, species-independent, and suitable for various applications such as monitoring gene expression, protein localization, and protein–protein interactions (e.g., yeast two-hybrid system, FRET experiments).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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