Thermo Fisher Scientific LC3B Monoclonal Antibody (GT3612)
LC3B 단백질을 인식하는 Mouse monoclonal 항체로, Western blot 및 Immunocytochemistry에 적합합니다. 자가포식(autophagy) 연구용으로 사용되며, 다양한 종에서 예측 반응성을 보입니다. Protein A로 정제된 액상 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT3612 |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787169 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Zebrafish (100%), Japanese Medaka (100%), Pig (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%)
- Positive Control: LC3B-transfected 293T, HepG2 (3 µM Thapsigargin treated for 12 hr), HeLa (50 µM chloroquine treated for 24 hr)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) is encoded by the human gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B).
LC3B is associated with microtubule assembly and plays an important role in neurogenesis and autophagy—the process of cytoplasmic component degradation.
Three human LC3 isoforms undergo post-translational modifications during autophagy. Macroautophagy, the main inducible pathway for cytoplasmic turnover, involves the formation of double-membrane autophagosomes that fuse with lysosomes for degradation.
LC3B mediates interactions between microtubules and cytoskeletal components, and is implicated in cancer, aging, metabolic, neurodegenerative, and cardiovascular/pulmonary diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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