Thermo Fisher Scientific alpha Actinin 2 Monoclonal Antibody (GT1253)
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Applications
Tested Dilution
Publications
Western Blot (WB)
1:500-1:3,000
Immunohistochemistry (Paraffin) (IHC (P))
1:100-1:1,000
Product Specifications
Species Reactivity
Human, Mouse
Host/Isotype
Mouse / IgG1
Class
Monoclonal
Type
Antibody
Clone
GT1253
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2.
Conjugate
Unconjugated Unconjugated Unconjugated
Form
Liquid
Concentration
1.27 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2787167
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (94%), Pig (99%), Chicken (98%), Bovine (99%).
Positive Control: HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it i involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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