Thermo Fisher Scientific alpha Actinin 2 Monoclonal Antibody (GT1253)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT1253

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.27 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2787167

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (94%), Pig (99%), Chicken (98%), Bovine (99%).

Positive Control: HepG2.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it i involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.