Thermo Fisher Scientific Nucleolin (Marker of Human Cells) Monoclonal Antibody (364-5, NCL/902)
인간 세포 마커 Nucleolin을 인식하는 Thermo Fisher Scientific의 단클론 항체입니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능하며, Human에 특이적 반응을 보입니다. 단백질 A/G 정제된 액상형 항체로, PBS buffer에 보존되어 있습니다.
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Thermo Fisher Scientific Nucleolin (Marker of Human Cells) Monoclonal Antibody (364-5, NCL/902)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 364-5, NCL/902 |
| Immunogen | Lysate of SU-DHL-1 nuclei (364-5); Recombinant human NCL protein (NCL/902) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Does not react with Mouse, Rat, or Cow.
Target Information
Nucleolin (NCL) is a eukaryotic nucleolar phosphoprotein involved in the synthesis and maturation of ribosomes. It is mainly located in dense fibrillar regions of the nucleolus. The human NCL gene consists of 14 exons and 13 introns, spanning approximately 11 kb. Intron 11 encodes a small nucleolar RNA termed U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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