Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), Brilliant Violet 421, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Available Formats

• APC
• Biotin
• Brilliant Violet 480
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Custom conjugation available

Product Specific Information

The monoclonal antibody CXCR3-173 recognizes mouse CD183 (CXCR3), a seven-transmembrane G-protein-linked chemokine receptor that binds CXCL9 (Mig), CXCL10 (IP-10), and CXCL11 (ITAC). CD183 plays a role in CD4 T cell responses to grafts, and knockout mice show compromised allograft rejection responses. Expression is found on NK cells, subsets of T lymphocytes and Tregs, and preferentially on Th1-polarized cells.

CXCR3-173 has been shown to affect chemotaxis in response to ligand; presence of ligand eliminates staining with the antibody. In vivo addition delays cardiac and pancreatic islet allograft rejection.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Optimal titration recommended.

Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is intended for use on cytometers equipped with a 405 nm violet laser. Ensure compatibility with your instrument.

When using multiple polymer dye-conjugated antibodies (e.g., Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

• Excitation: 407 nm
• Emission: 423 nm
• Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen.

Target Information

The CXCR3 gene encodes a G protein-coupled receptor selective for chemokines IP10, Mig, and I-TAC. These chemokines belong to the CXC subfamily and mediate leukocyte trafficking, integrin activation, cytoskeletal rearrangement, and chemotaxis. CXCR3 signaling involves Gi-type G proteins and phosphorylation-dependent receptor internalization, followed by recycling to the cell surface.

CXCR3 is prominently expressed in effector/memory T cells and inflamed tissues, suggesting involvement in inflammatory cell recruitment. It is a potential target for small-molecule antagonists in inflammatory disease treatment. Multiple transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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