Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), Brilliant Violet 421, eBioscience
마우스 CD183(CXCR3)을 인식하는 단클론 항체로, Brilliant Violet 421 형광체로 결합되어 유세포분석(Flow Cytometry)에 적합합니다. CXCR3 발현 T세포 및 NK세포 분석에 사용되며, 높은 특이성과 신뢰성을 제공합니다.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Brilliant Violet™ 421, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CXCR3-173 |
| Conjugate | Brilliant Violet™ 421 |
| Excitation / Emission Max | 406 / 423 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Available Formats
- APC
- Biotin
- Brilliant Violet 480
- FITC
- Functional Grade
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Custom conjugation available
Product Specific Information
The monoclonal antibody CXCR3-173 recognizes mouse CD183 (CXCR3), a seven-transmembrane G-protein-linked chemokine receptor that binds CXCL9 (Mig), CXCL10 (IP-10), and CXCL11 (ITAC). CD183 plays a role in CD4 T cell responses to grafts, and knockout mice show compromised allograft rejection responses. Expression is found on NK cells, subsets of T lymphocytes and Tregs, and preferentially on Th1-polarized cells.
CXCR3-173 has been shown to affect chemotaxis in response to ligand; presence of ligand eliminates staining with the antibody. In vivo addition delays cardiac and pancreatic islet allograft rejection.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Optimal titration recommended.
Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is intended for use on cytometers equipped with a 405 nm violet laser. Ensure compatibility with your instrument.
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
- Excitation: 407 nm
- Emission: 423 nm
- Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen.
Target Information
The CXCR3 gene encodes a G protein-coupled receptor selective for chemokines IP10, Mig, and I-TAC. These chemokines belong to the CXC subfamily and mediate leukocyte trafficking, integrin activation, cytoskeletal rearrangement, and chemotaxis. CXCR3 signaling involves Gi-type G proteins and phosphorylation-dependent receptor internalization, followed by recycling to the cell surface.
CXCR3 is prominently expressed in effector/memory T cells and inflamed tissues, suggesting involvement in inflammatory cell recruitment. It is a potential target for small-molecule antagonists in inflammatory disease treatment. Multiple transcript variants exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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