Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), Brilliant Violet 421, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• eFluor 660
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The eBioH4A3 monoclonal antibody reacts with human CD107a (LAMP-1), a highly glycosylated 110 kDa protein primarily located in lysosomal/endosomal membranes. CD107a is transiently expressed on the surface of degranulating cytolytic T cells and upregulated on activated platelets and some cancer cells.

• Applications Reported: Intracellular staining followed by flow cytometric analysis
• Applications Tested: Pre-diluted and tested for intracellular staining of Jurkat cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00)
• Recommended Use: 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is suitable for cytometers equipped with a 405 nm violet laser.

Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to reduce nonspecific interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

LAMP1 (CD107a) is a major lysosomal membrane component, with 1–2% found on the plasma membrane. It is a heavily glycosylated type I integral membrane protein involved in lysosome biogenesis and fusion with phagosomes. Upon cell activation, LAMP1 translocates to the plasma membrane via a tyrosine-based motif (YXXI). Alterations in this motif affect lysosome localization.

Cell surface LAMP1 and LAMP2 promote PBMC adhesion to vascular endothelium, suggesting a role in inflammatory cell recruitment. Increased LAMP1 expression is observed in neurons and glial cells near senile plaques in Alzheimer’s disease and in thymic involution.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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