Thermo Fisher Scientific Vimentin Recombinant Rabbit Monoclonal Antibody (VIM, 4388R)
Recombinant rabbit monoclonal antibody against human Vimentin (VIM, 4388R). Validated for WB and IHC applications. High purity (Protein A/G), liquid form, unconjugated. Suitable for research use in mesenchymal cell studies and cytoskeletal protein anal...
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- 7431-RBM7-P1ABX
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | VIM, 4388R |
| Immunogen | Recombinant fragment (aa366–466) of human Vimentin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Vimentin is a developmentally regulated intermediate filament protein found in cells of mesenchymal origin. It is involved in intracellular protein transport between the nucleus and plasma membrane. Vimentin expression occurs alongside desmin during early cellular development and is later replaced by tissue-specific intermediate filament proteins. It plays a role in steroid synthesis by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation by protein kinase leads to filament breakdown and cytoskeletal reorganization, facilitating cholesterol transport to mitochondria and enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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