Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Super Bright 436, eBioscience
Mouse CD4 단백질을 인식하는 GK1.5 클론의 Rat 모노클로날 항체로, Super Bright 436 형광체가 결합된 제품입니다. Flow cytometry에 최적화되어 있으며, 405 nm excitation / 436 nm emission 특성을 가집니다. T 세포 및 수지상세포 분석에 적합합니다.
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Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Super Bright 436, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 90 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host/Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GK1.5 |
| Conjugate | Super Bright™ 436 |
| Excitation/Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2662396 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV, Violet, and Yellow series
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue, Red, Violet, Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600, 645, 702, 780
- Request custom conjugation
Product Specific Information
The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays a crucial role in T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes, ≤0.125 µg/test (100 µL final volume)
- Excitation: 405 nm
- Emission: 436 nm
- Recommended Filter: 450/50 bandpass or equivalent
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD4 is involved in recognition of MHC class II molecules and acts as a co-receptor for HIV. It is primarily expressed in T helper cells but also found in monocytes, macrophages, and dendritic cells. Tissue-level expression includes thymus, lymph nodes, tonsils, spleen, and certain regions of brain and gut. CD4 facilitates T-cell activation through association with the T-cell receptor complex and Lck kinase and may mediate neuronal damage in infectious and immune-mediated CNS diseases. Multiple alternatively spliced transcripts are known (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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