Thermo Fisher Scientific CD4 Monoclonal Antibody (OKT4 (OKT-4)), Super Bright 436, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 10 publications

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• Brilliant Violet 650
• Brilliant Violet 786
• eFluor 450
• eFluor 660
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by most thymocytes, a subpopulation of mature T cells (T-helper cells), and at low levels on monocytes. CD4 acts as a receptor for HIV. The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block each other.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested using normal human peripheral blood cells. Recommended use is 5 µL (0.125 µg) per test in 100 µL final volume. Suitable cell number: 10⁵–10⁸ cells/test.

Fluorophore Information:
Super Bright 436 is excited with the violet laser (405 nm) and emits at 436 nm. Recommended detection filter: 450/50 bandpass or equivalent.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD4 is involved in recognition of MHC class II molecules and acts as a co-receptor for HIV. It is expressed in T-helper lymphocytes, monocytes, macrophages, and dendritic cells. CD4 is found in thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues such as brain and gut. It plays a role in T-cell activation via association with the T-cell receptor complex and protein tyrosine kinase Lck, and may mediate neuronal damage in immune-related CNS diseases.
(RefSeq, July 2017)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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