Thermo Fisher Scientific GM-CSF Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein
MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK

Preparation
Produced from sera of rabbits immunized with highly pure Recombinant Murine GM-CSF. The anti-Murine GM-CSF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA
To detect mGM-CSF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine GM-CSF (500-P65) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of recombinant mGM-CSF.

Western Blot
To detect mGM-CSF by Western blot analysis, use at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for recombinant mGM-CSF is 1.5–3.0 ng/lane under both reducing and non-reducing conditions.

Packaging
500-P65BT-1MG is provided as 2 × 500 µg.

Target Information

The GM-CSF gene encodes a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at chromosome region 5q31, associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in this cluster include interleukins 4, 5, and 13. The gene promotes tissue inflammation, and elevated cytokine levels, including GM-CSF, have been observed in SARS-CoV-2-infected patients with acute respiratory distress syndrome. Mice lacking this gene or its receptor develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.