Thermo Fisher Scientific Nestin Polyclonal Antibody
인간 Nestin 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합합니다. 줄기세포 및 신경계 연구에서 널리 사용되며, 항원 친화 크로마토그래피로 정제되었습니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 | - |
| Immunocytochemistry (ICC/IF) | 1:25 | 1 publication |
| Flow Cytometry (Flow) | 1:25 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to amino acids 1389–1416 of human Nestin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.35 mg/mL |
| Purification | Antigen affinity chromatography, Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C for short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2148923 |
Target Information
Nestin is a Class VI intermediate filament protein abundantly expressed in the developing central nervous system (CNS) and early embryonic neuroepithelial stem cells. It serves as a key marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin is also a superior angiogenic marker for evaluating neovascularity in tumors.
Highly specific antibodies to human Nestin are useful for characterizing progenitor cells differentiating into distinct lineages, enhancing the therapeutic potential of human neural stem/progenitor cells in CNS disease or injury treatment, and identifying neuroepithelial tumor cells.
Nestin expression has been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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