Thermo Fisher Scientific IKZF3 Monoclonal Antibody (OTI5E2), TrueMAB
IKZF3(Aiolos) 단백질을 인식하는 Mouse monoclonal antibody(OTI5E2)로, Western blot 및 IHC(P)에서 검증됨. Human 시료 반응성. 동결건조 형태로 제공되며, 재구성 시 1 mg/mL 농도 권장. B세포 발달 및 전사 조절 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5E2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–301 of human IKZF3 produced in E. coli. Target family: Aiolos (IKZF3); UniProt ID: Q9UKT9-1; Antigen range: 1–301 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, an additional desalting step is recommended (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The IKZF3 gene encodes Aiolos, a member of the Ikaros family of zinc-finger proteins. These hematopoietic-specific transcription factors (Ikaros, Aiolos, Helios) regulate lymphocyte development.
Aiolos plays a key role in B lymphocyte proliferation and differentiation and can form homo- and heterodimers with Ikaros to modulate chromatin remodeling and gene expression.
Multiple alternative transcripts encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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