Thermo Fisher Scientific CD34 Monoclonal Antibody (ICO115)
인간 CD34 단백질을 인식하는 마우스 단클론 항체. IHC(P) 및 유세포분석(Flow Cytometry)에 적합. 줄기세포 표지자로서 세포막 위치 단백질 검출에 활용. 항원 친화 크로마토그래피로 정제된 액상형 제품. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Flow Cytometry (Flow) | 0.5–5 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ICO115 |
| Immunogen | Recombinant protein of human CD34 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Kidney tissues
- Cellular location: Membrane
Target Information
CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) present on many stem cell populations. It serves as a stem cell marker, with reversible expression in human hematopoietic stem cells. CD34 may function as a surface receptor involved in receptor-mediated endocytosis and regulation of adhesion, differentiation, and proliferation of hematopoietic stem and progenitor cells.
CD34 expression indicates a specific hematopoietic developmental state, influencing adhesion properties and differentiation potential both in vitro and in vivo. It may act as an adhesion molecule mediating stem cell attachment to the bone marrow extracellular matrix or stromal cells, and as a scaffold for lineage-specific glycans, facilitating binding to lectins expressed by stromal or marrow components.
Additionally, CD34 may present carbohydrate ligands to selectins. The intracellular domain is phosphorylated by activated protein kinase C, suggesting a role in signal transduction. CD34 dysfunction is associated with diseases such as dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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