Thermo Fisher Scientific CD117 (c-kit) Monoclonal Antibody (2B8)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Positive Control: Jurkat cells
• Cellular Location: Membrane, cytoplasmic

Target Information

KIT (c-KIT) is a proto-oncogene and a type III transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. Together with its ligand, KIT regulates the growth and activation of various hemopoietic and non-hemopoietic cells.

Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Deregulation of KIT receptor TK by the prevalent activation loop mutation D816V is a key focus in therapeutic strategies targeting neoplastic mast cell growth.

c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract Cajal cells. Upon binding of stem cell factor (SCF), c-kit dimerizes, leading to receptor activation and autophosphorylation of tyrosine residues, including tyrosine 703 on the cytoplasmic domain. This modification enables Grb2 docking and activation of the Ras/ERK signaling pathway. SCF/c-kit can also activate PI3K, PLC-γ, and JAK/STAT pathways. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.