Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Brilliant Ultra Violet 805, eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Brilliant Ultra Violet 805, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: N/A

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 563, 737
• Brilliant Violet 421, 711, 786
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE, PE-Cyanine5, 5.5, 7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha).
CD127 complexes with CD132 (common gamma chain) to form the IL-7 receptor (IL-7R), a type I glycoprotein (75–80 kDa).
It is expressed by immature B cells, thymocytes, and most mature T cells with transient down-regulation upon activation.

IL-7 binding triggers signal transduction via tyrosine kinase pathways including Jak/STAT.
IL-7 is essential for lymphocyte development and homeostatic proliferation of peripheral T cells.
CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (T regs), and its reduction correlates with Foxp3 expression and suppressive activity.

Binding of eBioRDR5 to PBMCs is blocked by recombinant human IL-7 (cat. 14-8079).

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry at 5 µL (0.25 µg) per test (100 µL final volume).
Cell count range: 10⁵–10⁸ cells/test.

Brilliant Ultra Violet™ 805 (BUV805) emits at 797 nm and is intended for use with ultraviolet (355 nm) laser-equipped cytometers.
Use Super Bright Complete Staining Buffer or Brilliant Stain Buffer™ to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light.
Fixation: Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days at 4°C.
BUV805 tolerates short-term methanol fixation (<1 hour).
Excitation: 355 nm | Emission: 797 nm | Laser: Ultraviolet Laser

Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen.

Target Information

CD127 (Interleukin-7, IL-7) is a glycoprotein regulating lymphopoiesis.
The active receptor (IL7R) is an alpha/gamma chain heterodimer.
CD127 promotes proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+/CD8+ T cells.
Dysfunction is associated with severe combined immunodeficiency (SCID).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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