
Thermo Fisher Scientific DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (114F12C8), FITC, MonoRab
FITC로 표지된 DYKDDDDK(FLAG) 태그 인식 토끼 단클론 항체로, 높은 특이성과 친화도를 가짐. ICC/IF 및 유세포분석에 적합. 동결건조 형태로 제공되며, 재구성 후 0.5 mg/mL로 사용 가능. 단백질 태그 검출 및 정제 연구용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| Flow Cytometry (Flow) | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | Mammalian cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 114F12C8 |
| Immunogen | DYKDDDDK synthetic peptide coupled to KLH |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Purification | Protein A |
| Storage buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C or -80°C if preferred |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- Biotin
- HRP
- Custom conjugation available upon request
Product Specific Information
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRab™ technology to generate high-affinity and high-specificity monoclonal rabbit antibodies.
MonoRab™ DYKDDDDK Tag Antibody (FITC), mAb, Rabbit is specific to DYKDDDDK tags placed at C-terminal, N-terminal, and internal regions of fusion proteins.
This antibody improves the effectiveness of various immunoassays, aiding in the identification, detection, and purification of DYKDDDDK fusion proteins in bacterial and mammalian cells.
Target Information
The DYKDDDDK tag (FLAG tag) is a short peptide sequence used for protein purification, detection, and localization.
It consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, and is typically fused to the N- or C-terminus of a protein of interest.
This facilitates easy identification and isolation using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, including studies of protein-protein interactions, expression, and localization.
The FLAG tag’s hydrophilic nature minimizes protein denaturation or inactivation.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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