
Thermo Fisher Scientific DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (114F12C8), Biotin, MonoRab
DYKDDDDK 태그 단백질 검출용 고특이성 Biotin 결합 토끼 단일클론 항체. Western blot 및 ELISA에 최적화. MonoRab 기술로 높은 친화도 확보. 재조합 단백질 연구 및 정제에 적합. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 0.2–0.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | Mammalian cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 114F12C8 |
| Immunogen | DYKDDDDK synthetic peptide coupled to KLH |
| Conjugate | Biotin |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRab™ technology to generate high-affinity and high-specificity monoclonal rabbit antibodies.
MonoRab™ DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit is specific to DYKDDDDK tags placed at C-terminal, N-terminal, and internal regions of fusion proteins.
This antibody enhances the effectiveness of various immunoassays, aiding in the identification, detection, and purification of DYKDDDDK fusion proteins in bacterial and mammalian cells.
Target Information
DYKDDDDK tag (also known as FLAG tag) is a short peptide sequence used as a protein tag to facilitate purification, detection, and localization.
It consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys.
The tag can be fused to the N- or C-terminus of a protein, enabling easy identification and isolation using anti-FLAG antibodies.
DYKDDDDK tag is widely utilized in molecular biology and biochemistry for studies such as protein-protein interactions, expression analysis, and localization.
Its hydrophilic nature minimizes protein denaturation or inactivation compared to other epitope tags.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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