Thermo Fisher Scientific DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (114F12C8), HRP, MonoRab

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

-

ELISA (ELISA)

0.05-0.2 µg/mL

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Rabbit / IgG

Expression System

Mammalian cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

114F12C8

Immunogen

DYKDDDDK synthetic peptide coupled to KLH

Conjugate

HRP HRP HRP

View additional formats

• Unconjugated
• Biotin
• FITC
• Request custom conjugation

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.01% thimerosal

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRab™ technology to generate the high affinity and specificity monoclonal rabbit antibodies. MonoRab™ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit specific to DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of fusion proteins. The antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify DYKDDDDK fusion proteins in bacteria and mammalian cells.

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.