
Thermo Fisher Scientific DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (114F12C8), HRP, MonoRab
DYKDDDDK(FALG) 태그 단백질 검출용 HRP 결합 단일클론 항체. Rabbit 모노클론으로 높은 특이성과 친화도 제공. WB 및 ELISA에 최적화된 사용 농도. 단백질 정제, 검출, 위치 분석에 활용 가능.
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Thermo Fisher Scientific DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (114F12C8), HRP, MonoRab
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| ELISA | 0.05–0.2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | Mammalian cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 114F12C8 |
| Immunogen | DYKDDDDK synthetic peptide coupled to KLH |
| Conjugate | HRP |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
Additional Formats
Product Specific Information
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRab™ technology to generate high-affinity and specificity monoclonal rabbit antibodies.
MonoRab™ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit is specific to DYKDDDDK tags placed at C-terminal, N-terminal, and internal regions of fusion proteins. This antibody improves the effectiveness of various immunoassays, helping researchers identify, detect, and purify DYKDDDDK fusion proteins in bacteria and mammalian cells.
Target Information
DYKDDDDK tag (FLAG tag) is a short peptide sequence used for protein purification, detection, and localization.
It consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, often fused to the N- or C-terminus of a protein of interest.
The tag facilitates identification and isolation using anti-FLAG antibodies and is widely used in molecular biology and biochemistry research.
The FLAG tag’s hydrophilic nature minimizes protein denaturation or inactivation.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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