Thermo Fisher Scientific p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Recombinant Mouse Monoclonal Antibody (rKIP1, 1356)
Recombinant mouse monoclonal antibody against p27Kip1 for WB, IHC, ICC/IF, and Flow applications. High specificity and reproducibility. Suitable for cell cycle regulation studies. For research use only.
- 카탈로그번호
- 1027-MSM6-P1
- 판매단위
- pk
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Thermo Fisher Scientific p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Recombinant Mouse Monoclonal Antibody (rKIP1, 1356)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rKIP1, 1356 |
| Immunogen | Recombinant full-length human CDKN1B protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
p27 Kip1 functions as a negative regulator of G1 phase progression and may act as a mediator of TGFβ-induced G1 arrest. It is a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes and interacts with cyclin D-CDK4 complexes to modulate their activation. Depending on its phosphorylation state, p27 Kip1 can act as an inhibitor or activator of cyclin D-CDK4 complexes. The protein has an approximate molecular weight of 27 kDa, and its degradation via CDK-dependent phosphorylation and ubiquitination by SCF complexes is essential for the transition from quiescence to proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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