Thermo Fisher Scientific p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Recombinant Mouse Monoclonal Antibody (rKIP1, 1356)
p27Kip1 단백질을 인식하는 Thermo Fisher의 마우스 유래 재조합 단클론 항체입니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합하며, 고순도 Protein A/G 정제 방식으로 제조되었습니다. 세포주기 조절 연구에 유용합니다.
- 카탈로그번호
- 1027-MSM6-P0
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Recombinant Mouse Monoclonal Antibody (rKIP1, 1356)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rKIP1, 1356 |
| Immunogen | Recombinant full-length human CDKN1B protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
p27 Kip1 functions as a negative regulator of G1 phase progression and may mediate TGFβ-induced G1 arrest. It is a key regulator of the cell cycle, inhibiting cyclin E- and cyclin A-CDK2 complexes.
p27 Kip1 forms complexes with cyclin D-CDK4, influencing assembly and activation depending on phosphorylation state and stoichiometry.
With an approximate molecular weight of 27 kDa, degradation of p27 Kip1—triggered by CDK-dependent phosphorylation and ubiquitination by SCF complexes—is essential for the transition from quiescence to proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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