Thermo Fisher Scientific p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Recombinant Mouse Monoclonal Antibody (rKIP1, 1356)
Thermo Fisher Scientific의 p27Kip1 Recombinant Mouse Monoclonal Antibody (rKIP1, 1356)는 세포주기 조절 단백질 p27 Kip1을 검출하기 위한 고특이성 항체입니다. WB, IHC, ICC/IF, Flow Cytometry에 적합하며 HEK293 발현 시스템을 사용한 재조합 항체로 높은 순도와 안정성을 제공합니다.
- 카탈로그번호
- 1027-MSM6-P1ABX
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rKIP1, 1356 |
| Immunogen | Recombinant full-length human CDKN1B protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
p27 Kip1 functions as a negative regulator of G1 phase progression and may mediate TGFβ-induced G1 arrest. It is a key inhibitor of cyclin E- and cyclin A-CDK2 complexes and interacts with cyclin D-CDK4 complexes, influencing their assembly and activation. Depending on phosphorylation state and stoichiometry, p27 Kip1 can act as either an inhibitor or activator of cyclin D-CDK4 complexes.
This 27 kDa protein undergoes CDK-dependent phosphorylation and ubiquitination via SCF complexes, enabling the transition from quiescence to proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일명: 1027-MSM6-P1ABX_p27Kip1_P46527-1_House_mouse_PDP.jpeg)
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