Thermo Fisher Scientific CNOT4 Monoclonal Antibody (OTI2F10), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

CNOT4 is a 575 amino acid protein with E3 ubiquitin ligase activity, mainly involved in protein modification and ubiquitination. It contains:

• A C4C4-type RING zinc finger domain
• An RNA recognition motif
• A bipartite nuclear localization signal

CNOT4 is part of the CCR4–NOT (CAF1) core complex, functioning as a general transcription regulator that can both positively and negatively modulate transcription of various genes.
It interacts with CNOT1 via its C-terminus and with E2 ubiquitin ligases via the C4C4 RING domain.
Reports indicate that CNOT4 is phosphorylated in response to DNA damage at consensus sites recognized by ATM and ATR.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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