Thermo Fisher Scientific Phospho-PI3K p85/p55 (Tyr467, Tyr199) Polyclonal Antibody
PI3K p85/p55 단백질의 Tyr467 및 Tyr199 인산화 부위를 인식하는 rabbit polyclonal antibody. Western blot, IHC, ICC/IF에 적합하며, 인간, 마우스, 랫드 등 다양한 종에 반응. 액상 형태로 -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PIK3R1 (Accession P27986), corresponding to amino acid residues around phosphorylated Tyr199. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903051 |
Product Specific Information
Antibody detects endogenous levels of PI3K p85/p55 only when phosphorylated at Tyr467 (p85) or Tyr199 (p55).
The site Tyr467 was historically referenced as Tyr458.
Target Information
Phosphoinositide 3-kinase (PI3K, phospho PI3K) binds to activated (phosphorylated) protein-Tyr kinases through its SH2 domain and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
Phospho PI3K is necessary for insulin-stimulated glucose uptake and glycogen synthesis in insulin-sensitive tissues.
During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, coordinating cell growth, cell cycle entry, cell migration, and cell survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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