Thermo Fisher Scientific Phospho-PI3K p85/p55 (Tyr467, Tyr199) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PIK3R1(Accession P27986), corresponding to amino acid residues around phosphorylated Tyr199. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PI3K p85/p55,uniProtId:P27986-1,ncbiNodeId:9606,antigenRange:199|467,antigenLength:724,antigenImageFileName:PA5-118549_PI3K_p85p55_P27986-1_Rabbit.svg,antigenImageFileNamePDP:PA5-118549_PI3K_p85p55_P27986-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2903051

Product Specific Information

Antibody detects endogenous levels of PI3K p85/p55 only when phosphorylated at Tyr467(p85) or Tyr199(p55). The site Tyr467 was historically referenced as Tyr458.

Target Information

Phosphoinositide 3-kinase (PI3K, phospho PI3K) Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phospho PI3K is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.