Thermo Fisher Scientific Rabbit anti-Goat IgG F(ab`)2 Secondary Antibody, Biotin
Rabbit anti-Goat IgG F(ab`)2 Biotin 결합 2차 항체로 Western blot, IHC, ICC, Flow cytometry, ELISA, IP 등 다양한 응용 가능. Goat IgG 경쇄에 특이적이며 Fc 부분에는 반응하지 않음. 고감도 신호 증폭용으로 연구용에 적합.
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- 31753
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Thermo Fisher Scientific Rabbit anti-Goat IgG F(ab`)2 Secondary Antibody, Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:20,000–1:400,000 |
| Immunohistochemistry (IHC) | 1:500–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:5,000 |
| Flow Cytometry (Flow) | 1:200–1:1,000 |
| ELISA | 1:20,000–1:400,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Goat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1.3 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 15 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient |
| RRID | AB_228399 |
| Target | IgG |
| Antibody Form | Whole Antibody |
| Country of Origin | USA |
Additional Formats
- FITC conjugate
- HRP conjugate
- Custom conjugation available upon request
Product Specific Information
- Concentration may vary slightly between lots; refer to lot-specific datasheet for details.
- Product #31753 is validated for Western blot, IF, ICC, IHC, IP, and FACS applications.
- Reacts with light chains of goat IgG and common light chains of goat immunoglobulins, but not with Fc portion or non-immunoglobulin serum proteins.
- May cross-react with immunoglobulins from other species.
- Store at 4°C until opened. For extended storage, mix with equal volume of glycerol (final ~50%) and store at -20°C.
- Reconstitute with 1.5 mL distilled water (final concentration ~1.3 mg/mL, lot-dependent).
Target Information
Anti-Goat secondary antibodies are affinity-purified and specific for goat immunoglobulins. They are used for detection, sorting, or purification of target proteins. Secondary antibodies enhance sensitivity via signal amplification, as multiple secondaries can bind to one primary antibody. These antibodies are produced by immunizing host animals with pooled immunoglobulins and may be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) for high specificity.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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