Thermo Fisher Scientific Goat anti-Syrian/Armenian Hamster IgG (H+L) Secondary Antibody, Biotin

Applications

Tested Dilution

Western Blot (WB)

1:20,000-1:400,000

Immunohistochemistry (IHC)

1:500-1:5,000

Immunocytochemistry (ICC/IF)

1:500-1:5,000

ELISA (ELISA)

1:20,000-1:400,000

In Situ Hybridization (ISH)

Assay-dependent

Product Specifications

Species Reactivity

Hamster

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

1.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.8, with 3mg/mL BSA

Contains

0.08% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_228248

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Product # 31750 has been successfully used in Western blot, IF, ICC, IHC, ELISA and ISH applications.

Product # 31750 reacts with the heavy chains of syrian hamster IgG and with the light chains common to most hamster immunoglobulins, but does not react against non-immunoglobulin serum proteins. This antibody cross-reacts with horse IgG, human IgG, rat IgG, and swine IgG (3%), bovine IgG, cat IgG, dog IgG, mouse IgG, rat IgM, and Rhesus monkey IgG (1%) and it has minimum cross-reactivity with immunoglobulins from other species.

This anti-hamster IgG antibody conjugate recognizes IgG from both Syrian and Armenian hamsters.

Target Information

Anti-Hamster secondary antibodies are affinity-purified antibodies with well-characterized specificity for hamster immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.