Thermo Fisher Scientific Insulin/IRDN Recombinant Mouse Monoclonal Antibody (rIRDN, 805)

Applications and Tested Dilution

• Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL

Product Specifications

Product Specific Information

For immunohistochemistry on PFA-fixed tissues, incubate the antibody for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein, and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It promotes glycogen storage, triglyceride formation, and synthesis of proteins and nucleic acids.
Insulin is a 51-amino acid polypeptide derived from proinsulin, which is cleaved post-translationally into peptide A and peptide B linked by disulfide bonds and one molecule of C-peptide.
Deficiency of insulin results in diabetes mellitus, a major cause of morbidity and mortality. Insulin is also present in tumors of β-cell origin such as insulinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.