Thermo Fisher Scientific Insulin/IRDN Recombinant Mouse Monoclonal Antibody (rIRDN, 805)
Recombinant Mouse Monoclonal Antibody targeting Insulin for IHC applications. Validated for multiple species including human and mouse. Produced in HEK293 cells and purified by Protein A/G. Supplied as liquid form, unconjugated, concentration 1 m...
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Applications
- Immunohistochemistry (Paraffin) (IHC (P))
Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Pig, Rabbit |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rIRDN, 805 |
| Immunogen | Recombinant full-length human Insulin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein, and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids.
Insulin is a 51-amino acid polypeptide produced from proinsulin, which is cleaved into A and B chains linked by disulfide bonds and a C-peptide molecule.
Insulin deficiency leads to diabetes mellitus, a major cause of morbidity and mortality. It is also present in tumors of β-cell origin such as insulinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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