Applied Biosystems™

Axiom™ Multispecies 9 Genotyping Array, 384-HT format

The Axiom Multispecies 9 Genotyping Array is used for parentage verification and trait selection for honeybee, sunflower, rice, soybean, common자세히 알아보기

The Axiom Multispecies 9 Genotyping Array is used for parentage verification and trait selection for honeybee, sunflower, rice, soybean, common bean, bovine, ovine, equine, and canine species. It is a component of our Axiom Agrigenomics Genotyping solution where target genomic DNA is prepared and processed on the GeneTitan Multi-Channel instrument. The automated process include hybridization of the gDNA to this high-throughput microarray followed by staining, washing, imaging, and output of genotyping data for further analysis.

The Axiom Multispecies 9 Genotyping Array contains 48,440 SNPs selected for high-throughput production arrays based on genome coverage and best compromise to include the largest number of informative SNPs for each species. This array is also available in mini-96 format (Cat. No. 551417).

The Axiom Multispecies 9 Genotyping Array was designed under the Affymetrix Expert Design Program in collaboration with the Colombian Agricultural Institute (ICA), Agrosavia (Colombian Agricultural Research Corporation), Martín Ariel Cantore (Sunflower Breeder), Rosina Fossati (Genexa-Uruguay), Dr. Rosana Vianello, Dr. Claudio Brondani, along with research scientists connected to the Brazilian Agricultural Research Corporation (Embrapa) including Ms. Paula Valdisser (analyst) and Dr. Isabela Pavanelli (Post-Doc with grant from National Council for Scientific and Technological Development).

Array content
48,440 SNPs from the following species:
• Honeybee (Apis Mellifera): 12,000
• Sunflower (Helianthus): 10,763
• Rice: 8,875
--Including 4,029 SNPs from the Illumina bead chip CORNELL-IR_Rice_LD (1)
• Soybean (Glicine Max): 6,019
--Variety identification and genetic improvement
• Common Bean (Phaseolus vulgaris): 4,734
• Bovine: 2,463
--Trait selection, parentage identification with the ISAG core panel, GenoEx discovery panel and SNPs optimized for STRs imputation
• Ovine: 1,991
--Disease identification, markers for parentage, meat quality, markers for resistance and susceptibility to parasites, genetic conditions
• Equine: 989
--Disease identification across breeds, genetic conditions, ISAG parentage verification (Etalon and Tozaki) and 430 extra markers from Axiom Equine Genotyping Array (670K; Cat. No. 550583)
• Canine: 606
--Disease identification (>150 diseases across breeds), genetic conditions and ISAG core panel for parentage
--Contains markers from AgriSeq Canine Traits and Disorders Panel (Cat. No. A43406) and AgriSeq Canine SNP Parentage and ID Panel (Cat. No. A43407)

Applications
• Parentage analysis (bovine, equine, ovine and canine)
• Identification of economically important traits
• Genetic improvement
• Variety identification (soybean)
• Genome-wide association studies
• Genetic variability studies
• Molecular breeding: association mapping and genomic selection
• Trait introgression

Required products
GeneChip Command Console Software
GeneTitan Multi-Channel Instrument
Axiom Analysis Suite Software
Axiom Long Format Export Tool (AxLE)

References
1. Morales et al. 2020; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0232479

사양

어레이Genotyping

어레이 수384 arrays

형식384 HT-array Plate

수량1 plate

Unit Size1 plate