Thermo Fisher Scientific Human CCL25 (TECK) Recombinant Protein
인간 CCL25(TECK) 재조합 단백질로, CCR9 수용체 결합 활성 검증됨. Western blot, IHC, ELISA 등 다양한 실험에 사용 가능. E. coli 발현, 순도 98% 이상, 엔도톡신 <0.1 ng/µg. 동결건조 형태로 -20°C에서 장기 보관 가능.
- 카탈로그번호
- PHC1625
- 카테고리
- Protein
- 판매단위
- pk
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human CCL25 recombinant protein contains 127 amino acids |
| Molecular Weight | 14.2 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >98% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | Determined by binding the CCR9 receptor on the surface of MOLT4 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Carrier-Free
Reconstitution:
- Briefly centrifuge vial before opening.
- Reconstitute TECK in water to 0.1–1.0 mg/mL.
- Do not vortex.
- Store reconstituted solution at 2–8°C for up to 1 week.
- For long-term storage, dilute in buffer containing 0.1% BSA and store aliquots at -20°C to -80°C.
Storage:
- Lyophilized protein stable at room temperature for 1 month.
- For long-term storage, keep at -20°C.
- Working aliquots with carrier protein stable for ≥3 months at -20°C to -80°C.
- Avoid repeated freeze/thaw cycles.
Target Information
Chemokines regulate the trafficking of developing T cells within the thymus.
Chemokine C-C thymus expressed chemokine (TECK), also known as chemokine ligand 25 (CCL25), small inducible cytokine A25, chemokine b-15, or CK b-15, is primarily expressed in thymic dendritic and epithelial cells and the small intestine.
TECK, a CCR9 ligand, suppresses immature myeloid progenitor subsets stimulated by multiple growth factors.
It signals through CCR9, essential for thymocyte navigation.
Bone marrow pre-pro-B cells and IL-7/Flt-3 ligand-dependent pro-B progenitors migrate toward TECK, a response lost in later stages of B cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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