Thermo Fisher Scientific Mouse IgG1 Isotype Control (NCG01)

Applications

Tested Dilution

Western Blot (WB)

1:50-1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

Immunohistochemistry (Frozen) (IHC (F))

1:200-1:400

Flow Cytometry (Flow)

1 µg/mL

Control (Ctrl)

Assay-Dependent

Product Specifications

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Isotype Control

Clone

NCG01

Immunogen

Synthetic hapten which is normally not present in humans or animals

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10943239

Product Specific Information

MA5-14453 reacts in ELISA, flow cytometry, gel shift, immunofluorescence, immunohistochemistry (paraffin and frozen), immunoprecipitation, and western blot analysis.

Target Information

Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.