Thermo Fisher Scientific DNase I Polyclonal Antibody
Thermo Fisher Scientific의 DNase I Polyclonal Antibody는 인간 DNase I 단백질을 인식하는 토끼 다클론 항체로, Western blot, IHC, ICC/IF 등에 사용됩니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도의 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–282 of DNase I (Uniprot ID: P24855) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.19 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153259 |
Product Specific Information
- Recommended positive control: Molt-4
- Predicted reactivity: Dog (81%), Rabbit (81%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis.
DNase I, a Ca²⁺/Mg²⁺-dependent endonuclease ubiquitously expressed in mammalian tissues, mediates internucleosomal DNA degradation in human cells undergoing apoptosis.
DNase I is highly polymorphic, with at least six known alleles.
DNase II, an acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis.
DNase I cleaves DNA to 5′-phosphodinucleotide and 5′-phosphooligonucleotide end-products, whereas DNase II cleaves DNA to 3′-phosphomononucleotide and 3′-phosphooligonucleotide end-products.
DNase II is usually present in the cytoplasm of epithelial cells but concentrated in the nuclei of lens fibers, while DNase I is concentrated in nuclei of epithelial and fiber cells.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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